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| Id: | 21747
| | Autor: | Hanks, John H. | | Título: | Behavior of leprosy bacilli in complex liquid media with highly available sources of nutrient and acessory substances.
| | Fonte: | Int. J. Lepr;9(3):275-298, July-Sept. 1941. tab.
| | Resumo: | Completely homogenized suspensions of fifteen leprous nodules were inoculated into complex natural media such as blood, serum, leprous tissue extracts, chick embryo juice, and sterile egg yolk suspensions. These substances were supplemented with a variety of proteolytic digests, autolysates, peptones, products derived from acid-fast bacilli, yeast extract and simpler sources of nitrogen and carbon. Each inoculated medium also contained all of the nutrient substances present in the lepromata. Diphtheroid bacilli were recovered from three of six cases showing ulcers of the skin. One culture of a streptothrix and two of acid-fast bacilli were also obtained from one of the ulcerated cases. A third acid-fast microorganism was cultivated from a patient who showed no ulcers. These acid-fast cultures were not tubercle bacilli. By quantitative microscopic studies in liquid media from which aliquot portions were removed for comparison with control smears or formalinized suspensions, it was learned that the leprosy bacilli did not multiply in any of the 109 nutritional combinations tested, or in the presence of added carbon dioxide and oxygen. Growth did not occur in media shown to be suitable for the cultivation of human blood monocytes or of fibroblasts from lepromata. (AU).
| | Descritores: | MYCOBACTERIUM LEPRAE/isol
MEIOS DE CULTURA
| | Localização: | BR191.1 |
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